원문정보
초록
영어
In vitro organogenesis system of the valuable ornamental species, Prunus yedoensis which is native to Korea, was established through callus culture derived from root tissues. Callus were induced on the medium supplemented with 2,4-D and BA or NAA and kinetin. Organogenesis was differ from the callus type, and NAA and kinetin combination was effective to induce organogenic callus. Growth of callus was influenced by sucrose concentrations. High level of sucrose (over 5%) had adverse effects such as decreased fresh weight and increased mortality of callus. Shoots developed from the callus when GA3 was treated with BA in the medium. Results showed that GA3 is essential for shoot development and elongation from callus in this species.
목차
Introduction
Materials and Methods
Plant materials
Media and plant growth regulators
Results and Discussion
Callus induction
Effect of sucrose on embryogenic callus growth
Organogenesis from callus
Acknowledgement
References
