원문정보
초록
영어
The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazine.
목차
1. Introduction
2. Experiment Materials and Methods
2.1. Materials
2.2. Test materials
2.3. Experimental animals and induction of colitis
2.4. Measurement of ROS level in the serum
2.5. Preparation of cytosol and nuclear fractions
2.6. Immunoblotting analyses
2.7. Hematoxylin and eosin (H/E) stain of colon tissue
2.8. Statistical analysis
3. Result and Discussion
3.1. Effects of Ulcerative colitis (UC) and anti-inflammatory
3.2. Reactive oxygen species (ROS)
3.3. ROS overexpression activates MAPK including p38 and ERK1/2 and NF-κB
3.4. Pro-inflammatory
3.5. Apoptosis
5. Conclusion
Acknowledgments
References