earticle

영어

Pig organs have gotten the spotlight as precious resources which it acts a ‘bridge’ to connect organ donors with recipients of shortage organ problems. The cell mediated immune rejection must overcome to survive animal’s organ in human immune system for successful xenotransplantation. US11 protein originates a CMV and it is translocated MHC Class I molecules in endoplasmic reticulums. The known function of CD55(hDAF) is complement regulation protein which inactivates the c3 cleaving converttase. Also, it regulates released c3 by NK cell. In this study, we have analyzed the significance of US11 and CD55 expression for cell-mediated cytotoxicity. The pig ear fibroblasts obtained to establish transgenic pigs. And genotyping and gene expression confirmed by RT-PCR. Degeneration of MHC Class I evaluate each cell by FACS. Cytotoxicity of NK cell and CD8+ CTLs conducts 1:1, 2.5:1, 5:1 / 10:1, 25:1, 50:1 effector:target ratio, respectively. The expression of US11 has known as to attenuate MHC Class I to prevent cytotoxicity by NK cells and CTLs but our results indicate that make no difference expression of MHC Class I between wild type and US11 cell. Nevertheless, US11 gene products suppress to the interference of NK cells and CD8+ CTLs. In comparison between US11/CD55 cells with US11 cells, CD55 reveals to cause the synergistic effect with US11 gene in cytotoxicity of NK cell. The effect of CD55 expression was significantly interrupting cell lysis by NK cells and CTLs, these reactions are supposed to induce humoral immune activity by cytotoxic cells.