earticle

영어

Stem cells can be differentiated into numerous cell types and then can be used to research intractable disease and regenerative medicine. Induced pluripotent stem cells (iPSCs) are produced through an artificial manipulation in vitro, and have the advantage of being to customized to patient. In this study, we used the Sendai virus to produce porcine iPSC (p-iPSC) from Jeju Black Pig (JBP) cells. JBP cell was infected for 24 h with Sendai virus (1×104 cells/mL). One week after infection, they were spread onto mouse embryonic fibroblast (MEF) feeder cell layer. When we used JBP #4007 2nd passage cells for iPSC generation, primary colony was appeared between 3～4 weeks. The first and second colony were dissociated by treatment with trypsin and hereafter formed colony were mechanically dissociated by insulin syringe. During culture of the JBP-iPSC, some colonies were stained by alkaline phosphatase (AP) staining kit for confirming expression of the pluripotency marker. The stem cell markers as undifferentiation state, Oct4, Sox2, SSEA-1 and Nanog, were stained using JBP-iPSC at 5 passage. The JBP-iPSC colony have been maintaining in nowaday 14 passages, and the AP and immunostain were expressed. These results demonstrate that p-iPSC can derive from Jeju Black Pig cell using Sendai virus, and it will be used to stem cell research for animal disease model.