earticle

영어

Porcine pluripotent stem cells (pPSCs) have provided potentials for agricultural biotechnology and biomedical models. However, authentic pPSCs have not yet established because standards for pPSCs-specific markers and culture conditions are not clear. Therefore, we have generated various types of pPSC lines, porcine epiblast stem cells (pEpiSC type 1 and type 2) derived from in vivo derived epiblasts and porcine induced pluripotent stem cell (piPSC) lines derived from porcine fetal fibroblasts (PFFs) with lentiviral transduction of six reprogramming factors (hOct4, hNanog, hSox2, hc-Myc, hKLF4 and hLin28) or sendai viral transduction of four reprogramming factors (hOct4, hSox2, hc-Myc and hKLF4). As the results, Lenti viral piPSCs and pEpiSCs type 1 have showed AP positive but Sev viral piPSCs and pEpiSCs type 2 have showed AP negative. However, both AP positive and negative pPSCs have expressed pluripotent associated genes (Oct4, Nanog and Sox2). In addition, all of these cell lines have showed in vitro differentiation potentials. An increase of S and G2/M phase in pPSCs was shown when compared to that in the control (PFFs) based on cell cycle analysis. However, surface marker of SSEA-1 was expressed only in pEpiSCs type 2 and Sev viral piPSCs, whereas surface marker of SSEA-4 was expressed only in pEpiSCs type 1 and Lenti viral piPSCs. Additionally, Tra-1-60 and Tra-1-81 were positively expressed only in Lenti viral piPSCs. In conclusion, these results showed that it requires to demonstrate specific markers and culture conditions for pPSCs because criteria for pPSCs are not clear.