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Poster Presentation : Sperm / Male Reproduction

The Effects of Supplementation of BSA or Fatty Acid Free BAS on the Motility of Fresh or Cryopreserved Rooster Spermatozoa

초록

영어

This study examined factors affecting the analysis of motility of chicken semen. The viability of spermatozoa was estimated using varying dilution ratios and supplementation with BSA or fatty acid free (FAF)-BSA as protein sources in semen diluent. Fresh semen was examined after preparing dilutions in BPSE of 1/8, 1/16 and 1/32 at 25℃. The motility of incubated semen at each dilution was observed at 3 min (89.9%, 69.9 % and 53.2%), 30 min (86.7%, 71.4% and 51.7%), 1 h (89.5%, 74.0% and 53.5%) and 3 h (78.5%, 66.5% and 45.7%), respectively. The addition of BSA or FAF-BSA to BPSE diluent significantly increased the viability of semen in 1/32 dilution with results of 53.2% (control), 84.8% (BSA) and 92.9% (FAF-BSA) (p<0.05). This phenomenon was also observed in the dilution of frozen semen, where FAF-BSA treatment increased the viability of thawed semen from 17.6% to 34.0% in a 1/8 dilution (p<0.05). When the protein sources were used in the dilution, the survival rates of diluted chicken semen were also increased with time lapse. These results show that FAF-BSA may act to protect chicken semen and is suitable as a basic component of chicken semen diluent for the method of analyzing rooster semen after freezing.

저자정보

  • Sung Woo Kim Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea
  • Min Soo Kim Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea
  • Yeonhui Yu Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea
  • Chan-Lan Kim Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea
  • Ik Soo Jeon Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea
  • Chongdae Kim Poultry Science Division, National Institute of Animal Science, RDA, Pyungchang 25342, Korea

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자료제공 : 네이버학술정보

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