earticle

영어

The freezing semen extender of stallions has been used routinely for artificial insemination (AI) of the mares. The INRA96 was often used as a base solution for freezing extender for stallion semen in addition of egg yolk and glycerin. In this study, the possible use of SM, domestically developed semen extender for fresh and cool semen, as a base solution for freezing semen extender was tested. The semen was collected from 3 Thoroughbred stallions (an average age of 6.66±0.19) using CSU artificial vagina. Semen was diluted in SM or INRA96 at 1:2 ratio and centrifuged to remove the supernatant in where seminal plasma was placed. Sperms were resuspended with freezing medium prepared with 2% egg yolk and 2.5% glycerol added to SM or INRA96 and frozen in 0.5 cc straws. For thawing process, straws were immersed in the 37℃ water bath for 30 sec. After thawing, total and progressive motility, membrane integrity, viability and mitochondria membrane potential were measured to compare the freezing efficiency of these base solutions. Statistical analysis was performed using SAS program. Total and progressive motility, membrane integrity, membrane integrity, viability, and mitochondria membrane potential of frozen/thawed sperms with SM based freezing medium were not significantly different with those of sperms frozen/thawed with INRA 96 based freezing medium. This result suggests that SM is as effeiceint as INRA96 as a base solution for freezing medium. However, the membrane integrity (28±6.6), viability (22.8±3.8), and mitochondria membrane potential (24.6±11.8) of frozen/thawed sperms with SM were very low. Also total (32.3±4.2) and progressive motility (1.72± 0.52) of these sperms were not acceptable for commercial use. In conclusion, the efficiency of SM as a base solution for freezing medium of stallion semen is comparable with INR96.