earticle

영어

The feeding strategies are important to improve milk production, milk quality and cost-efficiently. It has been studies that the amount of concentrated fed should depend on the milk yield. However, there is no evidence of difference in colostrum composition based on different levels of feed. Here, we investigate the correlation between feeding levels and colostrum protein. We observed significant changes of colostrum protein between two groups, which were eating the standard diet and high-energy diet. Milk proteins, colostrum, were collected from the day of calving and 3rd day of calving. The change of milk protein was analysis using two-dimensional (2-D) gel electrophoresis and these proteins were identified using PMF analysis. Five protein were identified in milk from the day of calving. The level of αS2-casein precursor and β-casein were higher in the colostrum from high-energy diet group than standard-diet while, IgG3 heavy chain constant region, non-classical MHC class Ⅰ antigen isoform X2 and β-casein A2 variant were higher in colostrum of standard diet group. Twelve proteins were identified in milk from 3rd day of calving. All of these protein highly observed in colostrum from standard diet group than high-energy diet. In addition, Most of this protein known as immune-related factor and colostrum of standard diet group contain many of those factors than high-energy group, although we observed the mature milk yield is a slightly lower in the group of standard diet. On other hand, excess supply of diet can lead the milk production, which has less immune-related factor in colostrum.