earticle

영어

Present study, it is demonstrated that MMP-9 serum as well as follicular concentrations are related to a successful IVF resulting in pregnancy. Currently, a limited number of studies have investigated intrafollicular and serum MMPs, with inconsistent conclusions. However, MMPs theoretically could be important players in IVF processes. Moreover, different behaviors of MMP-2 and MMP-9 during the embryo development were confirmed; only MMP-9 has shown a vast difference between follicular and maturation oocyts. The main objective of this study is to examine the mode of MMPs activation during bovine in vitro fertilization. In particular, the relative employment of two major embryo culture medium in embryo development; e.g. ES(non-bovine serum) and CR(bovine serum) was compared. This results found that the level of MMP-2 and MMP-9, which degrades basal membrane, was different in the ES and CR in cultured at each stage of embryo development. In ES cultured system, MMP-9 was highly expressed throughout blastocyst inner cell mass and exhibited strong gelatinase activity. The MMP-9 expression continually increased as the embryo develops from oocyte maturation to fertilization, Meanwhile, the level of MMP-2 remained insignificant throughout these periods. However, decreased MMP-2 and 9 expression on the CR culture system. Interestingly, MMP-9 was actively expressed in ES culture system, inner cell mass is showing levels higher than trophoblast cell. Our result suggests a possibility that different subtype of MMP partakes major functions in the remodeling of inner cell mass in blastocyst. Such differences in MMP expression may impact to the distinct mode of follicular development in the two species.