earticle

영어

Fatty acid synthase (FASN) is an enzyme that catalyzes the synthesis of long chain fatty acid. During development, FASN plays a role in growth rather than the energy storage pathways. In this study, we hypothesis that knockout of FASN may affect the early embryonic development through induction of the endoplasmic reticulum (ER) stress. The function of FASN was studied using the Crispr/Cas9 technology. We found that FASN knockout induced ER stress by generation of reactive oxygen species (ROS), further resulted in the activation of adaptive unfolded protein response (UPR), caused the splicing of XBP1. In addition, FASN knockout increased ATF4 and CHOP expression, influenced phosphorylation of PERK. At last, Ca2+ was released from the ER and then taken up by the mitochondrial and influenced mitochondrial function, initiated apoptosis. These results demonstrated that FASN knockout induced generation of ROS, which mediated the activation of UPR via the ER and subsequent apoptosis in porcine early embryos.