earticle

영어

One of the most important issues in animal embryology is the ability of the oocyte to develop, that is, pluripotency. So, in this experiment, I want to get information about the early development of pluripotency by observing the presence of certain antibodies in the pluripotency. These results are expected to lead to positive results in many experiments including animal breeding and replication. In this experiment, CDX2, OCT4 and E-cadherin, among the many antibodies known to affect pluripotency, were observed for oocytes development in pigs. In mouse, CDX2 was found in late second stage development and OCT4 is known to be expressed specifically in the ICM region in blastocyst. E-cadherin was expressed in the junctional region of cells after the 2-cell stage. Blastocysts appeared in areas with junctions and cell walls. We observed the early stages of oocytes development in pigs based on PA (Parthenogenesis CDX2 and OCT4 were simultaneously imaged using an optical microscope in immunohisto chemical staining and compared to DAPI. E-cadherin was also experiment in the same way. In the development stage of oocyte development, CDX2 was expressed in blastocyst and expressed in nucleus except ICM (inner cell mass) region, and OCT4 was expressed in whole nucleus including ICM region in blastocyst. E-cadherin was expressed in the cell junctions and cell walls in the blastocyst. Our results showed that three antibody is pigs were expressed in pigs, as in mouses, and that they had an effect on pluripotency.