earticle

영어

Allicin (AL) is one of the chemical substance from garlic and has strong antioxidant activity and considered to represent anti-aging effect in vitro. The objective of this study was to investigate the effects of allicin treatment during porcine oocyte aging and their in vitro developmental competency after somatic cell nuclear transfer (SCNT). Porcine oocytes were maturated in vitro for 44 h (control), 44+24 h and 44+24 h with 0, 0.1, 1, 10 and 100 μM allicin (0, 0.1, 1, 10 and 100 AL, respectively). We investigated the effects of appropriate concentration of allicin on nuclear, cytoplasmic maturation and the developmental capacity of aged porcine oocytes. The 1 AL significantly increased maturation rate and normal spindle formation rate compared to control and 0 AL. The mRNA expression of cytoplasm maturation marker genes (MOS, BMP15, GDF9 and CCNB1) in 1 AL was higher than control and 0 AL. Allicin enhanced the rearrangement of abnormal spindle in aged oocytes. Parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryos development in 1 AL were significantly increased compared to others. Allicin treatment on aged oocytes improved their poor developmental capacity by rearranging the cytoskeleton. Thus, the allicin is effective agent to prevent the poor developmental competence of porcine aged oocytes and enhanced PA and SCNT embryo developmental capacity. Therefore, AL may be helpful for improve the aged oocytes quality to use on in vitro experiments.