earticle

영어

Poly(ADP-ribosyl)ation (PARylation) is related to DNA repair, chromatin modification, and apoptosis and catalyzed by poly (ADP-ribose) polymerases (PARPs). Recent studies have shown that PARylation affects preimplantation development and autophagy mechanism in pig embryos. However, the role of PARylation during in vitro maturation (IVM) has not yet been investigated. To study the effect of PARylation, cumulus-oocyte complexes were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during IVM. Nuclear maturation of oocytes were showed no significant difference in all stages (GV, MI, MII), but full expansion of cumulus cell was significantly decreased (11.05±1.09 vs. 48.40±0.67%) in 3-ABA treatment group. To evaluate mRNA levels of maturation and expansion related genes, we analyzed 3-ABA treated oocytes and cumulus cells. GDF- 9, BMP15, COX-2, PTX3 expression were upregulated but levels of HAS2, TNFAIP6 were downregulated in treatment group. To evaluate the effect of PARP inhibitior on embryo development and blastocyst quality, we experimented parthenogenetic activation (PA). As a results, the developmental rate of PA embryos was significantly decreased in 3-ABA group. Furthermore, TUNEL index (4.80±0.66 vs. 2.42±0.35) and total apoptotic index (6.44±0.81 vs. 3.08±0.51) was significantly increased, while Total cell number was decreased (41.12±2.10 vs. 50.38±2.27). Therefore, these results suggested that PARylation plays an important role in IVM of porcine oocytes.