원문정보
초록
영어
For successful pregnancy, the appropriate interactions between the maternal endometrium and the implanting conceptus are essential during the implantation period. In pigs, the implanting conceptus produces estrogens, interleukin 1 beta (IL1B), interferon delta (IFND) and interferon gamma (IFNG) during early pregnancy. Estrogens are produced by CYP19A1, a key enzyme for estrogen biosynthesis, and play a role in maternal recognition of pregnancy in pigs. IL1B is known to be involved in production and transport of prostaglandins in the endometrium. The role of IFND and IFNG during the implantation period is not fully understood. Also, localization of CYP19A1, IL1B, IFND and IFNG in the implanting conceptus is not fully understood. Thus, this study determined the localization of these molecules in conceptus during the peri-implantation period in pigs. We performed in situ hybridization and immunohistochemistry using conceptus on Days 12 and 15 of pregnancy. CYP19A1 were detected mainly in trophectoderm of conceptus on Days 12 and 15 of pregnancy. IL1B was expressed in trophectoderm of Day 12, but not Days 15. IFND mRNA and protein were localized to trophectoderm and endoderm of Days 12 and 15 with stronger immunostaining signal on trophectoderm than endoderm. IFNG mRNA and protein were mainly localized to trophectoderm, but not endoderm, on Days 12 and 15 of pregnancy. These results showed that CYP19A1, IL1B, IFND and IFNG were localized to conceptus, suggesting that these molecules secreted from conceptus during the peri-implantation period are dynamically regulated during conceptus development in pigs. Further study is needed to analyze the function of these molecules in the uterine endometrium during early pregnancy.