earticle

영어

S100 protein family is small calcium-binding proteins with EF-hand motif and comprises more than 20 proteins in human. S100 proteins are involved in intracellular and/ or extracellular signaling, such as proliferation, differentiation, apoptosis, calcium homeostasis, inflammation and migration. Previously, we have shown that the expression of S100A15A mRNA in the endometrium is increased on Day 12 of pregnancy compare to the estrous cycle. However, the expression and regulation of S100A15A have not been determined in the uterine endometrium during the estrous cycle and pregnancy in pigs. Therefore, we analyzed the expression and regulation of S100A15A mRNA in the uterine endometrium during the estrous cycle and pregnancy. Real-time RT-PCR analysis showed that S100A15A mRNA was expressed in the uterine endometrium during the estrous cycle and pregnancy with higher levels on Day 12 of pregnancy than the estrous cycle. To investigate the effects of steroid hormones and interleukin 1 beta (IL1B) on the expression of S100A15A mRNA, endometrial tissue explants from Day 12 of the estrous cycle were treated with steroid hormones, estradiol and progesterone, or IL1B. The levels of S100A15A mRNA were increased by estradiol and IL1B. These results showed that S100A15A was expressed in the uterine endometrium in a pregnancy stage-specific manner and the expression of S100A15A was regulated by estradiol and IL1B of conceptus origin, suggesting that S100A15A may play a key role in the establishment of pregnancy in pigs.