원문정보
초록
영어
Dynamic changes in the actin network are crucial for the cortical migration of spindle and establishment of polarity to ensure asymmetric division during meiotic maturation. Here, we show that Filamin A (FLNA) is an essential actin regulator that controls spindle migration and asymmetric division during oocyte meiosis. FLNA was localized in the cytoplasm and enriched at the cortex and near the chromosomes. Knockdown of FLNA impaired meiotic asymmetric division and spindle migration with a decrease in the amount of cytoplasmic actin mesh and cortical actin levels. Moreover, FLNA knockdown reduced the phosphorylation of Cofilin and Rho kinase (ROCK) near the spindle. Similar phenotypes such as decreased filament actin levels, impaired spindle migration and polar body extrusion were observed when active Cofilin (S3A) was overexpressed or ROCK was inhibited. Notably, we found that FLNA and ROCK interacted directly in mouse oocytes. Taken together, our results show that FLNA plays crucial roles in asymmetric division during meiotic maturation by regulating ROCK-Cofilin-mediated actin reorganization.