earticle

영어

DNA repair protein Rad51 homolog 1 (Rad51) plays a central role in Homologous recombination (HR) repair. HR depends on the formation of a Rad51 recombinase filament that facilitates strand invasion. However, whether Rad51 influences the porcine oocytes during maturation is unknown. The objective of this study is to investigate the expression and function of Rad51 during porcine oocytes maturing in vitro. Rad51 was mainly localized at the nuclear in the germinal vesicle (GV) stage and widely distributed in the cytoplasm form GV breakdown (GVBD) to MII stage. DNA damage inducing by the Etoposide was identified by the Rad51 foci formation which was localized with γH2AX. Inhibition of Rad51 increased the DNA damage, and induced metaphase I arrest with spindle defects, chromosomal misalignments and abnormal spindle assembly checkpoint (SAC) activity. Inhibition of Rad51 also increase ROS level and induced the abnormal mitochondrial distribution. Our results indicate that Rad51 play a critical role to maintain chromosome integrity as well as mitochondria activity during the porcine oocyte maturation.