원문정보
초록
영어
Spermatogonial stem cells (SSCs) are expected to undergo self-renewal and differentiation into different body cell types in vitro, which are expected to serve as a powerful tool and resource for transgenic animal technology. We have established an efficient isolating for SSCs from 1-day-old chicken testicular cells. This present study is aimed to carry out the culture and characterization of chicken SSCs. Disassociation of chicken SSCs were performed using two-step enzymatic digestion of type 4 collagenase and trypsin and were seeded in a twelve-well plate. In primary culture was observed colony forming on day four post incubation. In the subsequent passages, chicken SSCs were positive for alkaline phosphatase activity and showed positive expression for the transcription factor, Oct 4. Immunoblotting analysis revealed that the chicken SSCs showed expression for the GRP78. Therefore, our findings may have implications for developmental biology and regenerative medicine by using chicken as the transgenic animal model.