earticle

영어

Interferon-tau (IFNT) gene was originally recognized as as low molecular weight secretory protein produced by the conceptus during the peri-implantation period in ruminant ungulates. Two isoform interferon tau (IFNT) genes exist in bovine conceptus. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I bovine interferon tau (bIFNTc1) gene. However, studies of new type I bovine interferon- tau (bIFNTc1) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its transcription has not been elucidated. In the present study, Human choriocarcinoma JEG3 cells were co-transfected with an bovine IFNTc1 (–2.2-kb, –631-bp)-luciferase reporters (–2.2-kb, –631-bp-Luc) construct and several transcription factor expression plasmids. bIFNTc1 gene was had very effect on activity by alone ETS2, and/or AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNTc1 (–2.2-kb vs –631-bp)-luciferase reporters, expression level of the upstream region was not identified. The degree of transcriptional activation of the bIFNTc1(–2.2-kb)-luciferase reporter gene was had more effect by ETS2 and/or AP1, expression plasmid. These results indicate that an more ETS2 and/or AP1-binding site may be located between positions –2.2 kb to –631 bp of the bIFNTc1 gene. Also, transcription of the (–631-IFNT1c1)- Luc increased more than 30 times when the potential relationship between AP1 and ETS2, and their association with a co-activator, cAMP-response element binding protein- binding protein (CREBBP). These observation suggest that AP1 and ETS2 are the most probable binding partners for CREBBP in the potentiation of bIFNTc1 gene transcription. These results indicate that new type I bovine interferon tau (bIFNTc1) gene transcription is regulated by AP1 and ETS2, and suggest that AP1 and ETS2 could be a key molecule in determining bIFNTc1 gene transcription by the trophectoderm.