earticle

영어

Recombination activating gene-2 (Rag2) participate in the immune system process through V(D)J recombination of B-cells and T-cells activation process. The deletion of Rag2 gene impaired the immune regulation through complete loss of matured B-cells and T-cells. However, in this research we found that Rag2-ablated mice showed very marginal expression for both B-cells (CD20 and CD79a) and T-cells (CD4 and CD8) markers. These data indicated the possibility of leakiness of B-cells and T-cells at least in a marginal level in the spleen and thymus of Rag2-deficient mice. It also suggested that effectiveness of Rag2 deletion could be regulated based on the strain and age of the concerned mice. Thereafter, we investigated the miRNAs and genes expression profile of Rag2 knockout mice spleen and found that some of the deregulated genes are putative targets of the differentially expressed miRNAs and might be transcriptionally connected. Moreover, the deregulated miRNAs and genes are also actively involved in both B-cells and T-cells activation signaling network. Finally, we concluded that miRNAs along with the related genes are important regulator of B-cells and T-cells signaling cascade, and the Rag2 deletion might impaired B-cells and T-cells development in a miRNAs-dependent manner.