earticle

영어

The DJ-1 was found to be the first case that an oncogene also play a role for causative gene of neurodegenerative diseases. The DJ-1 is a multi-functional protein that play important role in tissue and participates in a number of intracellular signaling pathways. Especially, DJ-1 protein has a cellular protective role and promoted cell survival under an oxidative stress. However, the cellular protective mechanism of DJ-1 is not fully understand, and we needs to be further study their functions in canine. In the present study, we investigated the protective role of DJ-1 against induced oxidative stress, cell viability, cell proliferation assay, cellular apoptosis detection analysis, intracellular ROS were measured appropriately. The results showed that DJ-1 overexpressed cells were up-regulated cell viability under oxidative stress conditions, whereas loss of DJ-1 cells were down-regulated the cell survival activity. Additionally, overexpression of DJ-1 cells increased cell resistance to oxidative stress and inhibited the elevation of cell death and cellular ROS induced apoptosis. Moreover, DJ-1 overexpressed cells was increased mitochondrial functions by using confocal microscopy with MitoTracker staining. On the contrary to this, DJ-1 null cells show defective cellular protection and mitochondria activity against oxidative stress conditions. The data indicate that canine DJ-1 protein enhances the cellular survival activity and promote cell survival activity under the oxidative stress by attenuates cellular apoptosis and ROS generation.