earticle

영어

Porcine embryonic stem-like cells (pESLCs) are able to undifferentiated proliferation and self renewal. However, due to the lack of a system that can effectively culture pESLCs, it is difficult to long-term culture of pESLCs. Integrins composed of α and β subunits and αβ combination are molecular receptor acting on the cell surface when cells are bound to extracellular matrix such as fibronectin and collagen. Integrins regulate function of cells through the inside-out and outside-in signaling regarding motility, survival, differentiation and proliferation of cells. However, there is lack of information on integrins regarding to pESLCs. Therefore, the aim of this study was to confirm the presence of integrins in pESLCs through the integrins screening. Fluorescence immuno assay showed that integrin α1 and α2 were not expressed, whereas, integrin α3, α4, α5, α6, α7, α8, α9, αv and β1 were expressed in pESLCs. Also, fluorescence intensity showed that intensity of integrin α5, α7, and α8 was higher than twice compared to other groups (610.58±26.83; 419.69±13.84; 646.09±4.53; p<0.05), but, integrin α6 was lower intensity than other groups (50.55±2.65; p<0.05). To identify of integrin heteromiders subsequently, we experimented the cell attachment assay. Cultures with 10 μg/mL vitronectin and 60 μg/mL tenascin C showed significantly highest rate compared to controls, indicating the presence of integrin heterodimer α9β1 and αvβ1 on the membranes of pESLCs respectively (p<0.05). Therefore, integrin α3, α4, α5, α6 and α7 exist as subunits and integrin α9β1 and αvβ1 exist as heterodimers in pESLCs.