earticle

영어

Transplantation of male gem cells including spermatogonial stem cells (SSCs) in experimental animal models has been used to study SSC biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of male gem cells and the production of offspring. Here, effects of surgically induced cryptorchidism investigated in dog as a new recipient model for SSC transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity. The testes and epididymis were collected every week after the induction of artificial cryptorchidism (surgery) for one month. To determine the effect of surgical cryptorchidism, the seminiferous tubule diameter was measured and immunohistochemistry using PGP9.5 and GATA4 antibodies was analyzed. The diameters of the seminiferous tubules of abdominal testes were significantly reduced compared to those of the scrotal testes. Immunohistochemistry results showed that PGP9.5 positive undifferentiated spermatogonia were significantly reduced after surgical cryptorchidism induction, but there were no significant changes in GATA-4 positive sertoli cells. To evaluate the testis function recovery rate, orchiopexy was performed on two dogs after 30 days of bilateral cryptorchidism. In the orchiopexy group, functional sperm were detected, and it produced puppy. In this study, optimum experimental conditions and time provided for surgical preparation of a recipient canine model for SSC transplantation. Additionally, this data will contribute to recipient preparation by using surgically induced cryptorchidism in non-rodent species.