earticle

영어

In human organ transplantation area, the biggest problem is the shortage of donor organs. To solve this problem, xenotransplantation, such as pig-to-human, can be a solution. Despite the physiological and anatomical similarities in pig to human, the immune- rejection still remain a major barricade. The alpha-1,3-galactosyltransferase (GT) gene is responsible for synthesis of xenoreactive antigen galactose-1,3-galactose and many researchers have afforded in elimination of the xenoreactive antigen and producing genetically modified pigs for xenotransplantation. Despite of the effort, the problems in immune rejection are not entirely eliminated yet. Here, we constructed a vector expressing multiple human genes, hCD55, hCD39, hTFPI, and hC1 inhibitor, to reduce immune rejection and GT targeting vector. The two vectors were co-transfected into porcine ear fibroblast (PEF), and neomycin was used as a selective marker to isolate cells inserted the two vectors. We confirmed the TG cell lines by PCR and western blot analysis. Using established cell lines, we will produce the Knock-in pigs by SCNT. Also we expect that the cloned pigs carrying those multiple immune-modulation genes will overcome the current obstacles in xenotransplantation.