earticle

영어

Unlike allograft, xenotransplantation has problems such as hyperacute rejection (HAR), acute rejection and chronic rejection. HAR was rapid humoral immune responses against the pig carbohydrate Galα1,3-Gal epitope by human complement regulatory reaction, but HAR was almost overcome through knockout of α1,3-galactosyltransferase gene. The acute rejection is appeared within a few days to week caused by ischemia, diffuse intravascular coagulation and lack of cellular infiltrate. In this study, we bred the transgenic pigs containing US11 and hDAF (CD55) gene, evaluate transgene transmission ratio and efficiency of Reproduction rate in natural breeding. In addition, performed Cytotoxicity experiment about human NK cells and complement against each piglet ear fibroblast that have gene expression of US11, hDAF or both hDAF and US11. In breeding evaluate, sow has normal litter size, and piglets sex rate was followed 1:1. Transmission ratio of transgenes was indicated that wild type was 10%, US11 gene only was 20%, hDAF gene only was 20%, and both US11 gene and hDAF gene was 50% in 10 piglets. In cytotoxicity assay, US11 gene showed that effect to reduce cytotoxicity rate from human NK cell, and hDAF gene was significantly reduce the cytotoxicity ratio from complement. Besides, piglet cells that has both US11 gene and hDAF gene has effect to reduce cytotoxicity of NK cell and complement. In conclusion, our study showed that US11 and hDAF genes has effective to reduced Xenotransplantation rejection, and natural breeding was efficient to product transgenic pig.