earticle

영어

Aberrant expressions of Meg3 hamper the developmental potential of induced pluripotent stem cells (iPSCs) not only the birth of a chimera but also the generation of fully derived iPSC-derived clones by tetraploid complementation. This study was conducted to examine the effect of vitamin C (VC) on the quality of iPSCs in pig. Porcine iPSCs were generated from ear fibroblast cells using the SeV vector expressing 4 human factors (POU5F1, SOX2, C-MYC, and KLF4) with or without VC. Colonies emerged at Day 14 of transduction. Colony number of VC group was higher than that of control. Next, clonal lines of iPSCs were separately sub-cultured from every single colony under 2i (CHIR99021 and PD0325901)-LIF and MEF feeder culture condition and the expression of Meg3 and pluripotency-related genes (Pou5f1 and Lin28) were examined in each iPSC line. The expression level of these genes differed among iPSC lines. Porcine iPSCs that maintained self-renewal showed a high level of Meg3 as well as pluripotency-related genes expression. Whereas, porcine iPSCs with a low level of Meg3 could not maintain the typical self-renewal morphology over 10 passages. Our results suggest that the quality of porcine iPSCs could be improved by VC treatment, and the expression status of Meg3 may be closely related to the developmental potential of iPSCs in pigs.