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Poster Presentation : Sperm / Male Reproduction

Sperm Fertility and In Vitro Developmental Potential of Jeju Black Cattle Bull and Hanwoo Bull

초록

영어

The Jeju Black Cattle (JBC) are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. The breed’s qualities and genetic uniqueness helped it to be named as a protected, state-designated national monument in 2013. However, they are endangered crisis. Therefore, in vitro fertilization (IVF) technology is needed to preserve these endangered animals and to further improve their traits. In this study, we compared the sperm fertility and developmental potential of IVF embryos using Hanwoo sperm supplied by Korea Animal Improvement Association and JBC sperm made by Jeju Special Self-Governing Province Livestock bull semen Promotion Institute. [Hanwoo A, B (KPN) and JBC A, B grade]. Sperm was inseminated in 44 μL IVF drop contained 10 oocytes with sperm concentration of 1×106 cells/mL, and then 2 μL heparin and 2 μL PHE (20 μM penicillamine, 10 μM hypotaurine, 2 μM epinephrine) were added. At 2 days after IVF, the in vitro fertilization rate was significantly higher in Hanwoo-B sperm group (82.3±1.1) than in JBC-A sperm group (64.6±2.3) (p<0.05). The highest percentage of blastocyst formation was produced in Hanwoo-B sperm group (43.0±3.9) among four treatment sperm groups. The trophectoderm (TE) cell number was higher in Hanwoo-A sperm group (122±7.8) than in JBC-A sperm group (73±12.6). The inner cell mass (ICM) cell number of Hanwoo-A sperm group (46±2.5) was similar to that of JBC-A sperm group (46±4.0). Thus, the total cell number of day 8 blastocyst was higher in Hanwoo-A sperm group (168±8.9) than in JBC-A sperm group (119±15.9). These results demonstrate that there were different in sperm quality (viability and motility) between Hanwoo sperm (60∼70%) and JBC sperm (50∼60%), and their in vitro development capacity was also presented as difference. We need to embryo transfer study for field trial.

저자정보

  • Jae-Wook Yoon Jeju National University, Jeju National University Stem Cell Research Center
  • Seung-Eun Lee Jeju National University, Jeju National University Stem Cell Research Center
  • Hyo-Jin Park Jeju National University, Jeju National University Stem Cell Research Center
  • Sang-Gi Jeong Jeju National University, Jeju National University Stem Cell Research Center
  • Won-Jae Kim Jeju National University, Jeju National University Stem Cell Research Center
  • Chan-Oh Park Jeju National University, Jeju National University Stem Cell Research Center
  • Dong-Yong Hong Jeju National University, Jeju National University Stem Cell Research Center
  • Min-Jee Park Jeju National University Stem Cell Research Center
  • Eun-Young Kim Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio
  • Se-Pill Park Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio

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