원문정보
초록
영어
This study examined factors affecting survival rate of epididymal sperm from Korean native black goat using 0.25 mL straw. The sperm was cryopreserved by the height above 5 cm or 14 cm from the surface of liquid nitrogen (LN2) for 10 min and then plunged into the LN2. The motility of sperm after thawing was estimated using CASA system and movement analysis. The motile sperm rates after thawing at 14 cm freezing height was significantly higher than at 5 cm (85.3% vs. 73.0%). Also the hyper- activation rates showed that the more actively moving sperm counts appeared at 14 cm height experiment (55.3% vs. 47.6%). This phenomenon was also observed in the dilution of electro-ejaculted semen. So, these results show that testis originated sperm could be used for artificial insemination and fertility evaluation in vivo is needed to evaluate their contribution to animal genetic resource banks.