earticle

영어

The aim of the present study was investigated to determine optimal culture condition for IVP embryos using oocytes derived from follicles of ovum pick-up (OPU). Follicular oocytes were aspirated from Hanwoo native cows by the OPU method. After follicular aspiration, the number and morphology of cumulus-oocytes complexes (COCs) and the developmental competence of oocytes after in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture were evaluated. The quality of aspirated COCs did not differ among the individual donor. The rates of oocytes recovery from the follicles by ultasound-guided aspiration were not significantly different among individual, but the rate of oocytes useful for IVF was significantly higher (p<0.05) in cows (67%) than heifers (45%) The cleavage and the developmental rate to blastocysts was not significantly different between CR1aa and IVMD medium. In conclusion, the culture medium did not have any apparent effects on the quality of aspirated COCs and developmental competence of oocytes after IVM-IVF, but it may affect follicular recruitment in non-pregnant cows. Moreover, the reproductive phase may influence the developmental competence of the recovered oocytes.