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In the present study, we compared cauda epididymal sperm recovery methods in Hanwoo bulls by flushing and floating techniques. Fourteen testicles with epididymides were collected from 7 Hanwoo bulls (months of age=15.1±0.2, scrotal circumference (cm)=31.6±1.1, weight (kg)=391.1±16.6. Epididymis with vas deferens in each bull was isolated from testis and blood vessels on surface of cauda epididymis. To recover cauda epididymal sperm by flushing and floating, each testis was randomly selected from a pair of testicles. The mean weight, length, width, circumference of testicles were similar. For flushing method, a 26 gauge needle connected with 10 mL syringe was inserted into the duct of vas deference. About 12 to 15 incisions were made in cauda epididymis. Pre-warmed semen freezing medium (OptixCell, IMV, France) flushed and epididymal sperm were recovered. For floating method, cauda epididymis was cut and minced using blades in a 100 mm dish. Minced cauda epididymis tissues were incubated with 10 mL of semen freezing medium for 15 min at room temperature. Sperm suspension recovered by filteration with a cell strainer (100 μM nylon mesh). Sperm concentration adjusted to 40 to 50×106 cells/mL with semen freezing medium, semen dilution loaded to 0.5 mL straw and cryopreserved in liquid nitrogen tank. Frozen-semen was thawed at 37°C for 40 sec and measured sperm motility and motility parameters using a CASA system (sperm class analyzer, Spain). Five replicates were conducted in each experimental group of bull. Recovered semen volume (63.3±28.0 vs. 61.7±23.0 mL), motility (89.5±12.8 vs. 91.4±7.9%) among flushing and floating methods were not significantly different. In conclusion, the both of flushing and floating techniques can be used for cauda epididymal sperm recovery without difference of sperm quality.