earticle

영어

Fipronil is widely used pesticide for insects, such as granular turf products, pet care flea and tick solutions. Although the Fipronil presents moderately risky, only for several researches have been reported that the Fipronil affects fertility of insects, fish, and so on. However, effects of Fipronil on mammalian fertility are not fully understood yet. Therefore, the aims of this study are investigating the effects of Fipronil on sperm functions. Spermatozoa were incubated with various concentrations of Fipronil (0, 0.1, 1, 10, 100, and 300 μM). Then sperm motility and motion kinematics were evaluated using computer-assisted sperm analysis. In addition, capacitation status and intracellular ATP level were evaluated using combined Hoechst 33258/chlortetracycline fluorescence and ATP Assay kit, respectively. Sperm motility, rapid speed, progressive, curvilinear velocity, average path velocity, and mean amplitude of head lateral displacement were significantly decreased in higher concentration of Fipronil (1, 10, 100, and 300 μM) treatments. Beat-cross frequency was significantly decreased in presence of 10, 100, and 300 μM Fipronil. Straight-line velocity and the acrosome reaction were significantly decreased and increased in 100 and 300 μM Fipronil treatments, respectively. In addition, the highest concentration of Fipronil (300 μM) was significantly decreased capacitation status and intracellular ATP level. Our results showed that Fipronil suppress sperm motility, motion kinematics, the acrosome reaction, capacitation status and intracellular ATP level. Therefore, Fipronil can negatively affect male fertility and the concentration of Fipronil need to be controlled when Fipronil be used for pesticide.