earticle

영어

Temephos is recommended a non-systemic organophosphorus insecticide by WHO because it is safe for humans and has low toxicity to mammals compared with other chemical compounds such as chlorine, potassium permanganate, zinc carbamate, and dichlorodiphenyltrichloroethane (DDT). However, Temephos is involved in the hazardous pesticide category PAN Bad Actor (Pesticide Action Network, USA). Moreover, no study was performed to evaluate toxicity of Temephos in mammalian germ cell. The objectives of this study was to assess the effect of Temephos on sperm functions. First, caudal epididymis spermatozoa from ICR mice were incubated with Temephos (0, 0.1, 1, 10, and 100 μM). Then sperm motility and motion kinematics were assessed using computer-assisted sperm analysis. In addition, capacitation status and adenosine triphosphate (ATP) level were assessed using combined Hoechst 33258/chlortetracycline fluorescence and ATP detection kit, respectively. The acrosome reaction and capacitation status were significantly increased and decreased in the highest concentration of Temephos (100 μM), respectively. In addition, ATP levels in spermatozoa were significantly decreased in higher concentration of Temephos (1, 10, and 100 μM). However, Sperm motility and motion kinematics (motility, medium, slow, progressive, hyperactivated, curvilinear velocity, straight-line velocity, average path velocity, beatcross frequency, dance mean, amplitude of lateral head displacement) were not significantly altered with Temephos. The results were shown that Temephos affect capacitation status, the acrosome reaction and ATP level. Taken together, we suggest that the present study may be applied to evaluate risk of Temephos on male fertility.