earticle

영어

4-Octylphenol is final product derived from alkylphenol polyethoxylates that are used as a surfactant in rubber synthesis, or as a surface adhesive. In addition, Octylphenol mainly reaches the marine environment in waste waters form factories. Here, we were evaluated the effect of 4-Octylphenol for reproductive ability. We performed an in vitro study to comprehensively evaluate the toxicity of 4-Octylphenol on mouse testicular cells (TM4 Sertoli cells and TM3 Leydig cells) by examining the influence of 4-Octylphenol on the cell viability, cell death and apoptosis-related gene expression. The results obtained from the MTT assay showed that 4-Octylphenol suppressed both TM3 and TM4 cell viability in dose-and time-dependent manner. Moreover, the number of apoptotic and dead TM3 and TM4 cells was significantly increased after 4-Octylphenol. Based on this our result, we analyzed the expression of the apoptosis related gene in 4-Octylphenol exposed testicular cells (both TM3 and TM4 cells). The 4-Octylphenol significantly upregulated the mRNA levels of Bax, Bad, Bak gene and downregulated the Bcl-2 gene expression in dose-dependent manner. In conclusion, 4-Octylphenol could induce both TM3 leydig cells and TM4 sertoli cell apoptosis, and the cytotoxic effect of 4-Octylphenol on testicular cells may be associated upregulated apoptosis gene such as Bax, Bad and Bak, which is required for mitochondrial permeabilisation during apoptosis.