earticle

영어

As an one of detrimental effect during freezing and thawing process, fatty acids in plasma membrane of spermatozoa were released and sperm membrane was damaged. And several studies had reported that spermatozoa could absorb fatty acid in freezing extender. We expected that supplement of alpha-linolenic acid (ALA) during freezing process could improved efficiency of cryopreservation for boar sperm. Therefore, the aim of this study was to evaluate effect of ALA combined with Bovine Serum Albumin (BSA) and Methyl-β-Cyclodextrin (MβCD) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. For preparation of ALA-carrier protein complex, 3 ng/mL ALA was mixed with 0.7 μg/mL Bovine Serum Albumin (BSA) or 14 ng/mL Methyl-β-Cyclodextrin (MβCD) in distilled water. The boar semen was purchased from GUMBO Company. Boar semen was cryo-preserved in lactose-egg yolk (LEY) containing ALA, BSA, MβCD, ALA+BSA, ALA+MβCD and frozen sperm was thawed at 37.5℃ for 45 sec in water-bath. Sperm viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed sperm was increased in all treatment groups. However, there was no significant difference. Acrosome reaction and mitochondrial damage in frozen-thawed sperm was decreased in all treatment groups compared with control group. However, there was no significant difference. In all sperm characteristics, ALA+BSA treatment group was higher than all treatment groups. In conclusion, addition of ALA with carrier proteins during cryopreservation did not improves viability and acrosomal membrane and mitochondrial integrity in boar sperm. Therefore, it is necessary to continue the study on the effects of ALA with carrier protein on boar spermatozoa during cryopreservation.