earticle

영어

Embryo development is the most important time in the life of a multicellular organism because the limited cells of the embryo produce all the cells of the adult body. And transcription factors are transcriptional regulatory proteins that specifically bind to the transcriptional regulatory region DNA and activate or inhibit the transcription of that gene. It is change in the stage of embryo development that positions and levels of expression of transcription factors. Therefore, we know that transcription factors conduct vary crucial role in the stage of embryo development. So, if we could identify clearly changes of transcription factors, it can be very helpful in understanding the mechanism of embryo development. We experimented in transcription factors from GV oocyte to blastocyst how are expressed in porcine. We focused on especially pluripotency genes, which play an important role in early embryo development. So we conducted to immuno- staining using antibodies of Cdx2, Oct4, Sox2, Nanog, and E-Cadherin. Unfortunately we used mouse’s antibody because pig`s antibody against transcription factors did not exist. We checked five transcription factors (Cdx2, Oct4, Sox2, Nanog and E-Cadherin) in early embryo development in porcine. We found different expression patterns between mouse and pig in some stages. Therefore, we need to experiment that different patterns of transcription factors play a role in species, and why occur.