원문정보
초록
영어
The optimization of culture condition is both basic and important for improving oocyte maturation, subsequent development of preimplantation embryo, and further implantation. This study performed to confirm effect of pioglitazone (PIO) treatment directly on porcine oocyte during in vitro maturation, further development of embryo. Immature oocytes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, and 10 μM pioglitazone (0, 0.01, 0.1, 1, and 10 PIO, respectively) for 44 h. Although both oocyte survival and polar body emission did not significantly differ between the control and PIO-treated groups, percentage of normal spindle formation and chromosome alignment was increased in the 1 PIO-treated group than in the control group (control, 60.9±8.2%; and 1 PIO, 81.5±5.9%, p<0.1). Level of GSH was significantly increased in 1 PIO-treated group than in the control group (control, 100.0±5.0%; and 1 PIO, 119.8±5.2%, p<0.05), while the level of ROS was not differ in all groups. The rate of blastocyst was significantly higher in the 1 PIO-treated group than in the control at day 6 (control, 29.6±2.5%; and 1 PIO, 37.9±2.3%, p<0.05) and 7 (control, 35.1±1.6%; and 1 PIO, 41.8±2.7%, p<0.05), respectively. These results indicated that PIO treatment during IVM showing beneficial effects on oocyte and embryonic development. Pioglitazone will contribute to enhancing efficiency of in vitro production.