earticle

영어

Hesperetin (H), one of the citrus flavonoids, shows a various of pharmacological properties including antioxidant, anti‐inflammatory, and anticancer. The purpose of this study is to prevent in vitro aging by treating hesperetin to protect it from oxidative stress. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 0.1, 1, 10, and 100 μM hesperetin (aging, H-1, H-10, H-100 and H-250, respectively). The study investigated the effect of proper concentration of hesperetin on nuclear maturation rate, and ROS level, apoptosis proportion, and the developmental capacity of aging porcine oocytes. The H-100 group was the most effective of protect for oxidate stress. The all results of the investigated showed that the aging group was significantly worse than the control group. During in vitro aging, normal spindle formation, apoptotic proportion, and expression of maturation marker genes were protected by the H-100 group, that result then the H-100 group was similar to the control group. The ROS level was the H-100 group better than the aging group. The estrogen receptor gene was examined to determine how hesperetin enters the porcine oocytes. As a result, it was confirmed that estrogen receptor was used because the H-100 group was expressed more than the aging group. Thus, the hesperetin is effective agent to protect from the oxidative stress during in vitro aging of porcine oocytes. Therefore, hesperetin will improve the quality of mature oocytes by allowing in vitro experiments to be protected from oxidative stress.