earticle

영어

As an one of multifunctional cytokine, transforming growth factor-β (TGF-β) induces various signaling pathway in cells via bind to their receptor. This action of TGF-β plays a crucial role in regulation of uterine environment for successful pregnancy in mammalians. Plasminogen activators system (PAs) is one of important physiological system for reproductive events such as oocyte maturation, acrosome reaction, fertilization, and uterine remodeling. It is consist of two-types of PAs (urokinase-type, uPA; tissue-type, tPA), uPA-specific receptor (uPAR), and type-1 PA inhibitor (PAI-1). In various type of cells, activity of these system was regulated by gonadotropin, cytokines, and hormones. Although both of TGF-β and PAs system play important role in uterine environment, relationship between TGF-β and PAs system was not fully understood. Therefore, this study was conducted to investigate regulation of PAs activity and mRNA expression by TGF-β in porcine uterine epithelial cells (pUECs). pUECs were isolated from uterine horn and cultured until 50% confluence. Then, cells were starved in serum-free medium for 24 hours and subsequently medium supplemented with TGF-β supplemented medium (0, 0.1, 10, 50, and 100 ng/mL) for 24 hours. The expression level of mRNA was analyzed using real-time PCR and PA activity assay was used for measurement of PA activity in supernatant. In results, PA activity was significantly decreased in TGF- β 10, 50, and 100 ng/mL treared group compared with control group (p<0.05). On the other hand, TGF-β did not affect to expression levels of uPA, tPA, uPAR and PAI-1 mRNA. In present study, although expression of uPA, tPA, uPAR, and PAI-1 mRNA were not influenced by TGF-β treatment, relative PA activity was reduced according to TGF-β concentration increased. Therefore, these results suggested that TGF-β regulate translation process for control the PA activity in porcine uetrine cells.