earticle

영어

Understanding preimplantation embryo development is important because of early embryonic mortality during pre-and post-implantation in assisted reproductive technology (ART). Thus, assessment of in vitro produced embryo prior to ET is the most final in vitro step to monior embryo development and select the best embryo. However, diagnosis of early embryo developmental potential in ART is still limited. Tight junction (TJ) is a crtitical component for blastocyst formation including a fluid-filled cavity, but the functional role was not extensively elucidated after cavitation. To elucidated the importance, we employed loss of function studies and chemical treatment for disruption on interaction between TJs. In the knock-down (KD) or inhibition treated embryos (IHB), half of embryos were arrested between morula to blastocyst transition period. Moreover, among KD and IHB blastocyst transferred to uterus of recipients, half of them failed to maintain pregnancies after 2nd trimester. Collectively, TJ complex and its integrity provide insights into implication of translational research for prediction and selection of developmental competency in human ART.