earticle

영어

Apoptosis occurs under various developmental, physiological, and pathological conditions, including normal chicken cell death. We have previously shown that chGRP78 is typically expressed in chicken cells and plays a prominent role in the maintenance of cell homeostasis and prevention of apoptosis. In addition, chicken serum-mediated proliferation regulates chGRP78 to prevent apoptosis in chicken cells via chGRP78- mediated anti-apoptosis. However, the precise molecular mechanisms underlying the chGRP78-mediated protection against apoptosis remain undefined. Here, we performed a comparative proteomic analysis of control and chGRP78-overexpressing DF1 cells to elucidate the cellular events that are directly or indirectly regulated by chGRP78. Using 2D gel based proteomics and bioinformatics prediction analysis, we detected chGRP78 binding sites in AKT1-regulated proteins. chGRP78 promoted AKT1 activation, and chGRP78 silencing decreased AKT1 levels. Therefore, our findings have important implications for the maintenance of chicken fibroblast cells via the inhibition of apoptosis.