원문정보
초록
영어
Chicken embryo fibroblast (CEF) cells are widely used in the study of embryology and differentiation. CEF and mammalian fibroblast cells differ in their requirements for defined nutrients in cell growth conditions. However, the molecular mechanism of the induced CEF proliferation is poorly understood. Our previous research has demonstrated that chicken GRP78 deficient promotes chicken cell apoptosis. In additionally, these studies identified the chicken Peroxiredoxin 3 protein (chPRDX3) in chicken cells by chicken serum-induced proliferation following two-dimensional (2D) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analyses. We had confirmed a relation between chPRDX3 upregulaion and cell proliferation in chicken embryo fibroblast cells. Our data demonstrated that the chPRDX3 significantly enhanced fibroblast proliferation. Moreover, knockdown of the chPRDX3 using siRNA decreased fibroblast proliferation and increased apoptosis. Therefore, the chPRDX3 is required for the cell proliferation in chicken fibroblast cells. Based on these results, we suggest that the chPRDX3 signaling pathway plays a critical role in chicken fibroblast survival. Therefore, our findings have important implications for the maintenance of chicken fibroblast cells.