원문정보
초록
영어
Interferon-tau (IFNT) is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants. Also, multiple interferon (IFNT) genes exist in cattle, However, molecular mechanisms of these bIFNT genes whose expressions are limited have not been characterized. We and others have observed that expression levels of bovine subtype IFNT genes in the tissues of ruminants; thus, bIFNT1 and other new type I (bIFNTc1/c2/c3) gene co-exist during the early stages of conceptus development and non-trophoblast cells. Its genes transcription could be regulated through caudal-relatedhomeobox-2 (CDX2) and ETS2 and AP1(JUN) and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. Bovine earderived fibroblast (EF) cells, were co-transfected with luciferase reporter constructs carrying upstream (positions −1000 to +51) regions of bIFNT1 and other new type I (bIFNT c1/c2/c3) gene and various transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1/c2/c3-Luc increased when this constructs were co-transfected with CDX2, ETS2, AP1 and/or CREBBP. Also, Its genes was had very effect on activity by CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. However, the degree of transcriptional activation of the bIFNTc1 gene was not similar to that bIFNT1/c2/c3 gene by expression plasmid. Furthermore, RNA-seq analyses also revealed that the expression levels of bIFNT1/ c2/c3 gene mRNAs expression were highest on day 17, 20 and 22 trophoblast and Madin-Darby bovine kidney (MDBK), Bovine ear fibroblast (EF) and (Endo) non-trophoblast cells. But, bIFNTc1 mRNA had not same expression level, bIFNTc1 lowest levels than those of IFNT1/c2/c3 gene in both trophoblast and non-trophoblast cells. These results demonstrate that bovine subtype bIFNT genes display differential, in the trophoblast and non-trophoblast cells.