원문정보
초록
영어
The efficiency of the in vitro production of oocytes is significantly lower than that of in vivo culture. Allicin (AL) is generally known to regulate the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study performed to reveal the effects of allicin treatment on porcine oocyte maturation and developmental competence. Cumulus oocyte complexes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, 10, and 100 μM AL for 44 h. The rate of polar body emission was tended to be higher in the 0.1 AL-treated group than in the control group (p<0.1). The rates of cleavage and blastocyst formation significantly increased in the 0.1 AL-treated group (p<0.05). The reactive oxygen species level at metaphase II did not significantly differ among all groups. The expression of maternal marker (BMP15 and CCNB1) and anti-apoptotic genes significantly increased in the 0.1 AL-treated group at MII stage, respectively (p<0.05). The rate of phosphorylated p44/42 MAPK to p44/42 MAPK was ~4-fold higher in the 0.1 AL-treated group than in the control group (p<0.05). Consequently, these results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes. In conclusion, allicin enhances the efficiency of IVM to establish a novel IVC system, and we suggested that it will be helpful reagent as assisted reproductive technology (ART) in the future.