earticle

영어

In mammals, the uterus is the organ of pregnancy. In most mammals, conceptus implantation to the uterine endometrium consists of blastocyst hatching, migration, apposition/ attachment, invasion and subsequent placental formation. It is known that close to 50% of fertilized pre-implantation embryos in mammals, including humans, fail to implant. We hypothesized that there was the embryo quality-dependent expression of embryo development associated genes on Endometrial Epithelial Cells (EECs) of superovulated Hanwoo. Therefore examined to study the mode of EECs expression during pre-implantation. Additionally, we investigated how EECs respond to the presence of embryos by FSH and LH in bovine EECs in vitro(The EECs were divided into three different groups as follows good (n=4), fair (n=4), bad (n=2)). Our results found that the different expression of EST mRNA to each the group. In addition, it was found that the expression of genes related to MMP-9 and development showed different appearance in each group, and the result of embryo development was different. In particular, the expression of genes associated with the growth of cells from the Good group was very high, and the expression of genes affecting cell activity was also very high. However, Bad group had a high expression of Casp-3 protein and expression of metabolism-related genes of other cells was very low. Also, it is interesting that the expression of FSH and LH in the EEC of Good group is very high, and even in the evaluation of the development rate of in vitro fertilized egg using co-curture, the expression of genes related to cellular metabolic processes increases It was confirmed. These results suggest that FSH and LH and cellular action increase the metabolism of EEC and also increase the development of Embryo.