원문정보
초록
영어
Porcine skin and preserved cadaver skin are used for temporary wound coverage, but 1 to 2 weeks after grafting, these tissues undergo immune-mediated rejection. To avoid such antigenicity, different types of acellular dermal matrix (ADM) have been studied. The generation of pigs lacking the α-1,3 galactosyl transferase (GT—/—) expressed by α-Gal epitope (Galα1,3Galα 1,4GlcNAc-R) has minimized hyperacute rejection. This study was performed to investigate the expression of α-Gal epitope in the skin derived from GT—/— transgenic pigs. The skin (7/1,000 inches) was obtained by dermatome (Zimmer®Electric Dermatome) from around one month old of wild type (WT), GT—/—,and GT-MCP/-MCP piglets, respectively. The skins treated with decellularization were fixed, dehydrated, cleaned, and embedded. It was confirmed that α-Gal epitope was not detected in the skins from TG pigs stained with BS-IB4 lectin antibody, but in WT skin. To analyze the characteristics of immune response genes in ADM skins, the tissue sections were stained with anti-HLAE and anti-MHC I antibodies. Decellularized skins of GT—/—and GT-MCP/-MCP were not stained in both HLAE and MHC I, but the both immune response genes were strongly expressed in epidermis and dermis, respectively. Taken together, it can be postulated that ADM skin originated from GT KO pigs may be used as a source of xenoskingraft. Further studies are needed to evaluate the functions of the ADM skins of GT—/— and GT-MCP/-MCP pigs after xenograft.