원문정보
초록
영어
Mammalian spermatozoa must undergo several physiological modifications both prior to in vivo and in vitro fertilization. These functional/physiological modifications of spermatozoa, called capacitation, are prerequisites for fertilization. Therefore, sperm proteins that modify the aforementioned events could regulate their function and fertilization competence. Signal transducer and activator of transcription 1 (STAT1) is a transcriptional protein reported to have a role in sperm capacitation. However, the function of STAT1 in spermatozoa has not yet been clarified. Therefore, Our study was designed to evaluate the role of the STAT1 using the specific inhibitor, fludarabine (Flu) in mouse spermatozoa. In this in vitro study, mouse spermatozoa were incubated for 30, 60, and 90 min accordingly to investigate the role of STAT1 on sperm capacitation. Our results revealed that the expression levels of STAT1 was significantly decreased at 90 min, perhaps as a consequence of sperm capacitation. Simultaneously, we treated mouse spermatozoa with different doses of a specific inhibitor of STAT1, fludarabine (150μM, 1500μM). Although our results showed that motility/motion kinematics, viability, and capacitation status have no significant difference following exposure to fludarabine, capacitation status tends to increase. Taken together, it is tempting to speculate that STAT1 could be associated with sperm capacitation. However, further studies are needed to discover the underlying mechanisms of STAT1 on sperm capacitation.