earticle

영어

Bisphenol A (BPA) is an industrial chemical widely used to manufacture certain plastics. As a strong endocrine disruptor, BPA is capable of altering cell functions. Exposure to BPA correlated with reduction of motility and fertilization ability of spermatozoa by increasing oxidative stress. Since antioxidants are capable to protect cells from oxidative damage, it may minimize BPA-induced stress to cells. Here, we examine whether exposure of spermatozoa to antioxidants such as glutathione (GSH), vitamin C (Vit C), and vitamin E (Vit E) has any protective effects of BPA-exposed spermatozoa. In this in vitro trials, collected spermatozoa from sexually mature male mice were incubated with BPA and different antioxidants (Control, BPA, BPA+GSH, BPA+Vit C, BPA+Vit E, GSH, Vit C, Vit E) for 6 hours. The doses of BPA and antioxidants were chosen based on preliminary studies (BPA: 100 μM, GSH: 5 mM, Vit C: 100 μM, and Vit E: 2 mM). Following incubation, we detect the levels of reactive oxygen species (ROS) in spermatozoa as a measure of oxidative stress to the cell. Our results showed that antioxidants are capable to protect oxidative stress by ROS in BPA-exposed spermatozoa (p<0.05). Simultaneously, we examine motility parameters and capacitation status of spermatozoa by CASA, and CTC staining, respectively. Strikingly, antioxidants treatment of BPA-exposed spermatozoa was capable to restore sperm motility (p<0.05). However, hyperactivated sperm motility, motion kinematics parameters, and capacitation status of spermatozoa showed no difference between treatments groups. This findings evidence that antioxidants are capable to restore sperm functions from BPA mediated toxicity. Therefore, antioxidants can be considered as therapeutic agents to treat BPA toxicity. However, further studies are needed to confirm our preliminary findings.