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영어

The Jeju Black Cattle (JBC) are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. Jeju black cattle are smaller than other breeds and their disposition is very calm. The breed's qualities and genetic uniqueness helped it to be named as a protected, state-designated national monument in 2013. However, they are endangered crisis. Therefore, in vitro fertilization (IVF) technology is needed to preserve these endangered animals and to further improve their traits. In this study, we compared the sperm fertility and developmental potential of IVF embryos using Hanwoo sperm supplied by Korea Animal Improvement Association and JBC sperm made by Jeju Special Self-Governing Province Livestock bull semen Promotion Institute. [Hanwoo A, B (KPN) grade and JBC A, B two type]. Sperm was inseminated in 44 μL IVF drop contained 10 oocytes with sperm concentration of 1× 106 cells/mL, and then 2 μL heparin and 2 μL PHE (20 μM penicillamine, 10 μM hypotaurine, 2 μM epinephrine) were added. At 2 days after IVF, the fertilization rate was higher in Hanwoo sperm group (80.9±1.6) than in JBC sperm group (72.0±5.2). The IVF rate was significantly higher in Hanwoo-B sperm group (82.7±1.1) and JBC-B sperm group (84.5) than in JBC-A sperm group (67.0±5.1)(p<0.05). The blastocyst formation rate was the highest in Hanwoo-B sperm group (43.0±3.9) among four treatment sperm groups(p<0.05). The trophectoderm cell number was higher in Hanwoo sperm group (122±7.8) than in JBC sperm group (73±12.6). The inner cell mass cell number of Hanwoo sperm group (46±2.5) was similar to that of JBC sperm group (46±4.0). Thus, the total cell number of day 8 blastocyst was higher in Hanwoo sperm group (168±8.9) than in JBC sperm group (119±15.9). These results demonstrate that there were different in sperm quality (viability and motility) between Hanwoo sperm group (70∼80%) and JBC sperm group (60∼70%), and their in vitro development capacity was also presented as difference. We need to more sperm fertility study using JBC sperm.