earticle

영어

Porcine uterine ex vivo model is a useful tool for investigating gamete maturation, activation, fertilization and early embryonic development. This study was to evaluate fertilization ability of in vitro matured (IVM) porcine oocyte (MII) using porcine uterine ex vivo model. IVM-MII oocytes were fertilized with 7.5×107, 15×107 and 30×107 sperm for 20min in porcine uterine ex vivo model. And, the oocytes were then flushed and performed in vitro culture (IVC). IVM-MII oocytes used for in vitro fertilization (IVF) served as control 1. Before IVF, IVM-MII oocytes cultured in porcine uterine ex vivo model for 20min served as control 2. In the results, it was found that the sperm penetration rate in the control 2 (73.0±4.2) was significantly higher than the other treatment group: 7.5×107 (29.7±4.4), 15×107 (34.3±3.2) and 30×107 (44.3±7.4). But there was no significant difference between control 1 and control 2. Monospermic penetration rate in all treatment groups was significantly higher than control groups, and polyspermy rate in all treatment group was also significantly lower than control groups. These results suggested that porcine uterine ex vivo model improved monospermic penetration by reducing sperm penetration ratio. In the further study, it is required to evaluate the embryonic development, GSH and ROS accumulation levels of MII oocytes fertilized using porcine uterine ex vivo model.