earticle

영어

Growth differentiation factor8 (GDF8) is a member of the transforming growth factor-β that has been identified as a robust physiological regulator. According to recent studies, the GDF8 is detected in oviduct fluid and uterus which led us to suggest that the GDF8 may effect on preimplantation embryonic development and act paracrine role to correlate with successful late-blastocyst implantation in in vivo. We investigated the effect of GDF8 supplement during in vitro culture (IVC) of porcine embryos derived from in vitro fertilization (IVF) on cleavage and blastocyst (BL) formation rate, and gene transcription level analysis in BL. Data were analyzed by one way ANOVA, followed by Tukey’s range test. Respectively 0.2, 2 and 20 ng/mL of GDF8 were added during IVC, and experimental groups were as follows; control (0), 0.2, 2, and 20 GDF8 supplement groups. At IVC 48hr, there was no significant difference on cleavage rate from the different concentration of GDF8 supplement groups (65.7%, 66.0%, 66.3%, and 65.8%, respectively). After additional 120hr of embryo culture, 0.2 group was shown significantly (p<0.05) higher than control in blastocyst formation rate and total cell number (32.5% and 88.0±7.3 VS 40.4% and 118.4±12.7, respectively). Using the achieved IVF BLs, the specific gene expression pattern were evaluated. The embryo development competence marker Pcna, Pou5f1 and Sox2 (1.81, 2.85 and 2.09 times, respectively), and cell junction assembly regulator Adam10, Adam17, Tjp1, Cdh1 (1.40, 1.98, 1.79 and 1.80 times, respectively) genes mRNA transcript levels in 0.2 group were significantly increased in 0.2 group compared with control. Moreover, pro-apoptotic factor Cas3 gene mRNA transcript levels was significantly decreased in 0.2 group than control (0.62 times). In conclusion, the supplementation of 0.2 ng/mL GDF8 during IVC significantly improved embryonic developmental potential via regulating embryo developmental competence markers and cell junction assembly regulator transcriptional levels.